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The size, PDI, and zeta potential of the samples were measured using a Malvern Zetasizer (Brookhaven Instruments, Holtsville, NY) at 25 °C based on the principles of dynamic light scattering (DLS). Samples were typically 200 nmol liposomes in 1 mL of HBS solution (10 mM HEPES, 150 mM NaCl, pH 8.0).
The morphology of liposomes was observed using a transmission electron microscope (TecnaiG220, FEI, Hillsboro, OR, USA). The liposomal solution was dropped onto a carbon-coated copper grid followed by drying in air, and the sample was then subjected to observation.
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