Mouse neuropathology

DD David W. Donley
MR Marley Realing
JG Jason P. Gigley
JF Jonathan H. Fox
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Mice designated for stereological analysis were perfused with 4% paraformaldehyde and treated as described [34,35]. Briefly, fixed brains were sectioned at 40 μm on a freezing microtome, and serial sections were collected in 12-well plates so that one well contained every 12th brain section. Sections from one well were mounted and thionin stained. The Cavalieri method and nucleator methods in StereoInvestigator (MicroBrightField) software were used to determine striatal volume and neuronal cell body volume, respectively.

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