For induction of HF, rats were injected subcutaneously with ISO for two successive days at different doses (85 and 170 mg/kg, respectively) (Mohamed et al., 2015). Only animals exhibited electrocardiographic deviations (ST elevation) and high serum creatine kinase-MB 24 h after the 2nd ISO injection, were chosen to complete the study.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.