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Creatine concentration was measured by using Creatine Assay Kit (Abcam, UK) following the manufacturer’s instructions. For cell samples, cells (2 × 106) were collected and washed with cold PBS. For tumor tissue samples, appropriate amount of tissue was harvested and washed with PBS. Then, the supernatants were deproteinized, mixed with reaction mixture and incubated at 37 °C for 1 h. The optical density (OD 570 nm) representing creatine concentrations was detected by EON spectrophotometer (BioTek, USA). The total amount of creatine was normalized to cell numbers.
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