Cell migration analysis

THP-1 (pretreated with 100 ng/mL PMA) and RAW264.7 cells were stimulated using 100 ng/mL LPS for 6 h, after which they were incubated with DHA or PBS for 24 h. The supernatant was added to the lower chamber. A cell suspension (4 × 10⁵) was seeded into the upper chamber. The cells were maintained at 37 °C in a 5% CO₂ incubator for 12 h, fixed in ethanol, and stained with crystal violet. Migrating cells in 4 random fields were counted, and their images captured using a digital camera.