Human phospho-RTK antibody array

HL Heng-Huan Lee
YW Ying-Nai Wang
WY Wen-Hao Yang
WX Weiya Xia
YW Yongkun Wei
LC Li-Chuan Chan
YW Yu-Han Wang
ZJ Zhou Jiang
SX Shouping Xu
JY Jun Yao
YQ Yufan Qiu
YH Yi-Hsin Hsu
WH Wei-Lun Hwang
MY Meisi Yan
JC Jong-Ho Cha
JH Jennifer L. Hsu
JS Jia Shen
YY Yuanqing Ye
XW Xifeng Wu
MH Ming-Feng Hou
LT Lin-Ming Tseng
SW Shao-Chun Wang
MP Mei-Ren Pan
CY Chin-Hua Yang
YW Yuan-Liang Wang
HY Hirohito Yamaguchi
DP Da Pang
GH Gabriel N. Hortobagyi
DY Dihua Yu
MH Mien-Chie Hung
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Proteome Profiler Human Phospho-RTK Array Kit (R&D Systems, #ARY001B) was used to detect the potential activation of RTK signals by hRNase 1 treatment. All procedures were performed according to the manufacturer’s instruction. Briefly, capture antibodies for specific RTKs were spotted in duplicate onto nitrocellulose membranes, prepared with the kit. Cell lysates (600 µg) were incubated with the array membrane at 4 °C overnight. After washing away unbound material, proteins in cell lysates containing phosphorylated tyrosine residues bound to the capture antibodies of RTKs were detected by a pan anti-phospho-tyrosine antibody conjugated to HRP. Last, the binding signal was measured by using chemiluminescent detection reagents and ImageQuant LAS 4010 (GE Healthcare).

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