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Western blotting analysis.

ZC Zirong Chen
WN Wei Ni
JL Jian-Liang Li
SL Shuibin Lin
XZ Xin Zhou
YS Yuping Sun
JL Jennifer W. Li
ML Marino E. Leon
MH Maria D. Hurtado
SZ Sergei Zolotukhin
CL Chen Liu
JL Jianrong Lu
JG James D. Griffin
FK Frederic J. Kaye
LW Lizi Wu
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Tissues or tumors were processed for protein isolation after snap freezing in liquid nitrogen. Protein extracts were prepared and subjected to immunoblotting as previously described (32). The following antibodies were used: anti-MAML2 TAD (catalog 4618), anti–phospho-RB (Ser807/811, catalog 8516), anti–phospho-EGF receptor (Tyr1068, catalog 3777), and anti-EGFR (catalog 2232) from Cell Signaling Technology; anti-FLAG (catalog PAI-984B) from Thermo Fisher Scientific; anti-RB (catalog 554136) from BD Biosciences; anti–β-actin (catalog 5316) from Sigma-Aldrich; anti-CDK4 (catalog sc-260), anti-CDK6 (catalog sc-177), and anti-P16 ( catalog sc-56330) from Santa Cruz Biotechnology; and anti–β-tubulin (catalog 1799-1) and anti-GAPDH (catalog 2251-1) from Epitomics. The protein bands on Western blots were quantified by using ImageJ software (NIH).

Copyright and License information:  ©2021 Chen et al.
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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