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Cellular uptake of PSs

PV Pablo Vallecorsa
GV Gabriela Di Venosa
MB M. Belén Ballatore
DF Dario Ferreyra
LM Leandro Mamone
DS Daniel Sáenz
GC Gustavo Calvo
ED Edgardo Durantini
AC Adriana Casas
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The LM3 cells were exposed to different concentrations ranging from 0.15 to 5 μM of PSs in medium without serum during 3 or 24 h. The concentration ranges to perform intracellular PS accumulation profiles have been chosen based on MTT cytotoxicity assays (see Figure S1 and Table S1). After 3 consecutive washes with PBS, the PSs were extracted from the cells employing DMSO and afterwards, fluorescence was measured in a Perkin Elmer LS55 fluorimeter employing the maxima excitation and emission light wavelengths (Table ​(Table1).1). Standard solutions of PSs dissolved in DMSO were employed. The cellular uptake of the PSs per 105 cells was determined from fluorescence calibration curves constructed by plotting the peak height of the PSs standard solutions. The calibration was linear within the employed range.

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