The Construction of Nude Mouse Tumor Formation Model

Four-week-old female BALB-c nude mice were provided by Shanghai Lingchang Experimental Animals Co., Ltd (Shanghai, China). The animal experiments were approved by the Medical Ethics Committee of People's Hospital of Deyang City. Xenograft models were constructed using shCtrl and shEIF3B SW1990 cells. The shCtrl and shEIF3B SW1990 cells in logarithmic growth phase were digested by trypsin and suspended into cell suspension. Then, the cell suspension was resuspended by D-Hanks or PBS, and the density of the cells reached 2 × 10⁷ cells/ml. After that, 200 μl of cell suspension (4 × 10⁶ cells) was injected subcutaneously into mice (10 mice/each group). The Vernier caliper was used to measure five times the volume of tumor during the feeding period. After 38 days, the mice were sacrificed, the tumors were removed, and the weight of the tumors was measured. Before being sacrificed, 0.7% sodium pentobarbital at the dosage of 10 μl/g was injected intraperitoneally for several minutes, and the fluorescence was observed by the in vivo imaging system (IVIS Spectrum, Perkin Elmer). Tumor tissues were frozen in liquid nitrogen and stored at −80°C.