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Total RNA from the rat PVN was extracted out and reverse-transcripted into cDNA (AT341, TransGen, Beijing, China). Primer synthesis: MST1-F: 5′-GCTGCGGCATCAAATCA-3′; MST1-R 5′-TGGAAAGGGTGCGAGTG-3′; YAP-F: 5′-TCGTCATGGGTCTAGTTGG-3′; YAP-R: 5′-GATGTGGC GGAGTTTCAG-3′; Actin-F: 5′-TCAGGTCATCACTAT CGGCAAT-3′; Actin-R: 5′-AAAGAAAGGGTGTAAAACGCA-3′. Then real-time quantitative PCR was performed (SG Fast qPCR Master Mix B639273, Sangon, Shanghai, China).
Copyright and License information: ©2021 Zhu, Zhu, Hou, Chen, Qu, Zhang, Li, Wang, Chen, Lv, Wang, Zhang and Hou.
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