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Ferric-reducing antioxidant power (FRAP) assay

MA Muhdin Aliye
AD Aman Dekebo
HT Hailemichael Tesso
TA Teshome Abdo
RE Rajalakshmanan Eswaramoorthy
YM Yadessa Melaku
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The ferric reducing antioxidant power assay of the extracts and isolated compounds were measured according to Birasuren et al.24. The FRAP reagent was prepared by mixing acetate buffer (25 mL, 0.3 M), TPTZ (2,4,6-tripyridyl-s-triazine) solution (2.5 mL), and FeCl3∙6H2O solution (2.5 mL), and then heated to 37 °C before use. A properly diluted sample (0.1 mL, 100 µg/mL) was mixed with FRAP reagent (4.0 mL) to form a mixture. This was incubated at 37 °C for 10 min in the dark, and the absorbance was measured at 593 nm against a blank (distilled water). The results were expressed as aqueous solution of ferrous sulfate (FeSO4∙7H2O), and derived from a calibration curve of the standards. Likewise, the FRAP value of ascorbic acid was obtained by the same procedure.

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