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RM-1 and basal epithelial cells (1:1) were co-incubated in the same 6-well plate for experiment group while only RM-1 was cultured for control group. The mono-layer cells were scratched in the middle of well plate with 200 µl pipette tips and washed with PBS to remove floating cells. PBS was replaced by (DMEM)/F12 without FBS. Images were photographed under microscope at 0 h and 24 h, respectively. Photos were quantified with Image J and the width of 24 h subtracted the width of 0 h for the migrated width.
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