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Peripheral blood mononuclear cells were isolated using the Ficoll-Histopaque technique (Sigma Aldrich, St. Louis, MO, United States) within 4 h of blood collection. Total RNA of PBMCs was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, United States) following the manufacturer’s instructions, and then dissolved in DNase/RNase-free water and stored at –80°C.
Copyright and License information: ©2021 Ma, Xie, Zhang, Yang, Jiang, Ouyang, Zhao, Liu, Xu and Li.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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