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Mice at 4–5 months of age were placed in the conditioning chamber (Coulbourn instruments) and, after a 2-min adaptation period, received three foot shocks (2 s, 0.5 mA) at 2, 3, and 4 min. After the foot shock, mice remained in the chamber for an additional minute and then were returned to their home cage. After 24 h, mice were placed back into the same context for 5 min, and freezing was monitored. Data acquisition and analysis were measured using FreezeFrame (Coulbourn Instruments).
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