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Eca-109 cells were cultured and grown in 12-well plates at 5 × 104 cells/well. 24 h later, the medium was replaced with fresh medium containing 2 μM compounds and incubated for an additional 24 h. The cellular uptake of the compound was determined by a fluorescence spectrophotometer according to the method previously reported40.
Cellular uptake of the conjugates was observed using a fluorescence microscope. Cells were cultured over coverslips. After 24 h, 2 µM of each conjugate was added and incubated overnight and then observed under a microscope41.
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