Flour From Treatment 3 (FT3)

Only the shell was separated from the cob (by hand), the whole corn cob was cooked in a cooking pot (10 L) with water at boiling temperature (96 ± 2°C) for 3 h. After that time, water was drained, the grain was removed, cobs were cut crosswise into 2 mm thick pieces and dried at 45°C/24 h in a convection oven. After that, they were ground in a mill with a 1 mm-mesh (Thomas Wiley Model 4).

The flours obtained from the three treatments (FT1, FT2, FT3) were stored in clean and dry jars for further analysis. The cooking water was kept in closed sterile jars in order not to generate residues as much as possible. It was frozen at −20°C until use.