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Dansylcadaverine (MDC) and LysoTracker Red Staining
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Cells were seeded in 24-well plates at a density of 1×104 cells/coverslip and treated as described in phase A. Then, cardiomyocytes were washed with prewarmed PBS and incubated with fluorescence dye MDC (Beyotime, Shanghai, China) or LysoTracker Red DND-99 (Invitrogen™, Oregon, USA) in darkness for 30 min at 37 °C. After that, images were captured by a fluorescence microscope (Olympus, Japan).
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