Mitochondrial morphology staining using MitoTracker Red CMXRos

To observe the morphology of mitochondria, MitoTracker Red CMXRos (Thermo Fisher) was used to stain worms using a previously published protocol [17]. The dye was added in seeded NGM plates under the dark condition at least 1 day before the experiment. A Nikon Eclipse Ti confocal microscope coupled with a Zyla-4.2 camera (Oxford Instruments) was used to take images of stained mitochondria in the anterior intestine cells.