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TUNEL assay for cardiomyocyte apoptosis quantification
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Cardiomyocyte apoptosis was evaluated by Terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) positive nuclei colocalized with DAPI staining with the use of in situ cell death detection kit. Percent of the number of TUNEL-positive apoptotic cells divided by the total number of DAPI stained cells (nucleated cells) was determined as the apoptosis index as described in previous studies [14, 45, 46].
Copyright and License information: ©2021 Bo-Htay et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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