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Before virus infection, the viral RNA (vRNA) copy numbers corresponding to MOI 0.5 were calculated by RT-qPCR, as described above [22]. HEK-293 cells were infected with the DENV2 strain 16681 at MOI 0.5 in the medium, which contained 6 µM of PF-429242 or the same amount of DMSO. At 96 h p.i., the supernatant was collected, and the vRNA was quantified by RT-qPCR. The harvested-DENV corresponding to the MOI 0.5 was used to continuously infect the next HEK-293 cells and so forth. A total of 5 sequential passages were conducted.
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