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Vesicle Leakage Assay

CM Christian Mink
ES Erik Strandberg
PW Parvesh Wadhwani
MM Manuel N. Melo
JR Johannes Reichert
IW Irene Wacker
MC Miguel A. R. B. Castanho
AU Anne S. Ulrich
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The peptide-induced lipid vesicle leakage was determined using a fluorescence-based assay as described previously in detail (Grau-Campistany et al., 2015). Leakage of ANTS/DPX from DMoPC/DMoPG (1:1), POPC/POPG (1:1), DErPC/DErPG (1:1), or POPE/POPG (1:1) vesicles was determined at a range of peptide concentration. Vesicle solution was added to a peptide solution in buffer at pH = 7.5, and the fluorescence signal was followed for 10 minutes. The data was scaled to the signal after addition of Triton-X, which gave the value for 100% leakage. Measurements were repeated at least three times.

Copyright and License information:  ©2021 Mink, Strandberg, Wadhwani, Melo, Reichert, Wacker, Castanho and Ulrich
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