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Oil Red O staining and quantification
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The cells were fixed with 3.5% formaldehyde solution for 10 min, rinsed twice with phosphate-buffered saline (PBS), and incubated with 1.5% Oil Red O in 60% isopropanol (O0625; Sigma-Aldrich) for 1 h. The cells were washed three times with PBS and dried to observe lipid droplets. For quantification of differentiated cells, stained Oil Red O was dissolved in dimethyl sulfoxide and its absorbance was measured at 510 nm.
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