Liquid chromatography

YY Ying Yu
PT Panli Tan
ZZ Zhenchao Zhuang
ZW Zhejiong Wang
LZ Linchao Zhu
RQ Ruyi Qiu
HX Huaxi Xu
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Nano LC-UltiMateTM 3000 RSLC nano System; two Column Mode: Trap Column (Acclaim PepMap C18, 3 μm, 100 Å, 75 μm × 2 cm), Analytical Column (Acclaim PepMap C18, 2 μm, 100 Å, 75 μm × 25 cm); mobile phase: A: 0.1% formic acid in water; B: 0.1% formic acid in 80% acetonitrile; gradient: 3–8% B in 6 min, 8–30% B in 102 min, 30–100% B in 8 min, 100–100% B in 4 min; flow rate: 300 nL/min.

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