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KEGG and GO enrichment pathway analyses of differentially expressed genes(DEGs)by Metascape and DAVID

JD Jialin Dai
QZ Qiong Zhang
CW Changwu Wan
JL Jiangjin Liu
QZ Qiaojun Zhang
YY Yanni Yu
JW Jie Wang
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For the genes corresponding to the differentially expressed proteins (DEPs) after screening, we used various databases to carry out functional enrichment of related differentially expressed genes (DEGs) to explore their possible influence on a specific aspect of the disease. DAVID, (an online analysis tool (https://david.ncifcrf.gov), used to provide biofunctional comprehensive and systematic annotation information for a list of large-scale proteins or genes), was used to perform KEGG and GO annotations pathway analysis of DEGs. It is mainly used for the enrichment analysis of the function and pathway of the genes of DEGs [17].The enrichment analysis was visualized by using ggplot2 from R. Then we used the Metascape database (https://metascape.org/gp/index.html) for GO enrichment analysis and KEGG pathway enrichment analysis again [18]. A P-value < 0.05 was considered statistically significant.

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