Prior to experiment, the abdominal skin of SD rats was shaved and cleaned to eliminate autofluorescence interference of hair and all rats were fasted overnight with free access to water. Rats were randomly divided into two oral groups and two injection groups with three animals in each group. The rats in oral groups were received QT-HNCs with particle size of 280 and 550 nm intragastrically at dose of 90 mg/kg, while QT-HNCs with particle size of 280 and 550 nm were given to the rats in injection groups by injection via caudal vein. Fluorescence images of animals were captured at predetermined time points by the IVIS System under anesthesia with isoflurane.
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