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Cells pellets were fixed in 70% ethanol, stained with PI/RNase Staining Buffer (BD Biosciences, San Jose, CA, USA) for 10 min, and then analyzed with a FACSCalibur flow cytometer (BD Biosciences). The acquisition count was set to 20,000 events for each sample. The nuclei population in each phase of the cell cycle was determined and analyzed, as described previously (13).
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