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In vivo wound healing assay

JZ Jilai Zhao
JP Jatin Patel
SK Simranpreet Kaur
SS Seen-Ling Sim
HW Ho Yi Wong
CS Cassandra Styke
IH Isabella Hogan
SK Sam Kahler
HH Hamish Hamilton
RW Racheal Wadlow
JD James Dight
GH Ghazaleh Hashemi
LS Laura Sormani
ER Edwige Roy
MY Mervin C. Yoder
MF Mathias Francois
KK Kiarash Khosrotehrani
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Full thickness excisional wounds were created using a 6 mm sterile punch biopsy (Stiefel Laboratories, Research Triangle Park, NC) along the dorsal skin. Wounds were then left open with animals culled at each respective timepoints after wounding. In lineage tracing experiments, animals were injected with tamoxifen prior to wounding. Wound closure and total surface were manually defined, with the surface area recorded at individual timepoints. Measurements for the surface area were calculated using ImageJ software (National Institute of Health). To assess fibrosis and scar formation, large full thickness excisional wounds (1.5 × 1.5 cm) were created. The wounds were left to heal for 28 days before the scar tissues were harvested and measured for scar area using ImageJ.

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