Gene expression analysis with nanoString

CD14⁺ monocytes were isolated from frozen PBMCs of either naive RRMS patients or patients in the CLAiMS trial, whereas only monocytes from naive RRMS patients were incubated in the presence or absence of CLA-mix (75 µM per isomer) for 24 h. RNA was isolated using RNeasy^® Plus Micro Kit (Qiagen) according to the manufacturer’s instructions. Measurement of the nCounter^® Human Myeloid Innate Immunity Panel was performed with the nCounter^® FLEX Dx (NanoString Technologies) at the Division of Translational Pathology, Münster. Quality control, normalization, and differential expression analysis were performed with nSolver Analysis Software 4.0 (Advanced Analysis Tool). Undetectable genes were excluded from further analysis. Adjustment of P-values was performed according to the Benjamini-Yekutieli correction.