4.6. Cellophane Membrane Penetration Assays

To test the penetration ability of each strain, hyphal blocks from each strain were inoculated on the sterile cellophane membrane overlaid on PDA plates. The cellophane membrane was cut into a size of 3 × 3 cm. Plates were cultured at 25 °C for 2 days (Pre). At 2 dpi, the entire membrane with colony was removed, and the resulting plates were cultured at 25 °C for 2 days (Post). This experiment was performed with three biological replicates and five technical replicates for each treatment.