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NIT-1 Cell Culture
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The mouse pancreatic β-cell line NIT-1 was purchased from ATCC (no. CRL-2055) and cultured in Ham’s F12K medium supplemented with 10% fetal bovine serum at 37°C in a humidified incubator with an atmosphere of 5% CO2. The cell culture medium was exchanged every 48 hours. The NIT-1 cells were harvested and passaged by detaching, aspirating and separating the adherent cells, followed by incubation with cell dissociation buffer (an enzyme-free Hanks’-based solution).
Copyright and License information: ©2021 Chang, Lin and Chen
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