Three-Chamber Sociability and Social Novelty Test

TG Talia E. Gileadi
AS Abhyuday K. Swamy
ZH Zoe Hore
SH Stuart Horswell
JE Jacob Ellegood
CM Conor Mohan
KM Keiko Mizuno
AL Anne-Katrine Lundebye
KG K. Peter Giese
BS Brigitta Stockinger
CH Christer Hogstrand
JL Jason P. Lerch
CF Cathy Fernandes
MB M. Albert Basson
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Individual mice were tested in the three-chamber box over three trials in a protocol adapted from Yang et al. (2011). In trial 1, the mice were habituated to the empty box over 10 min. In trial 2, an unfamiliar sex-matched juvenile conspecific mouse was placed in a wire containment cup on either the left or right chamber (counter balanced within each group), and an unfamiliar mouse-like object (tally counter, Appleton Woods GC101) was placed in a wire containment cup in the opposite chamber. Test mice could explore the box for 10 min. Social preference was measured as 100×(time spent with novel mouse)/(time spent with novel mouse novel object). In trial 3, the unfamiliar object was replaced with a novel unfamiliar conspecific, and the test was run for another 10 min. Social novelty preference was measured as 100×(time spent with novel mouse)/(time spent with novel mouse familiar mouse).

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