Eleven ccRCC cell lines (MZ1257RC, Caki1RC, MZ1790RC, MZ1795RC, MZ2861RC, MZ1846RC, MZ2733RC, MZ1851RC, MZ1774RC, MZ1973RC, MZ2905RC) were previously established from primary ccRCC and cultured according to Seliger et al. (32). The MZ1257RC cell line was used in all functional studies and cultured in DMEM media, 10% FCS and L-Glutamine. The human embryonic kidney cell line HEK293 was cultured in DMEM F12 media and 10% FCS. All cell lines were cultured at 37°C at 5% CO2.
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