MPTP-Induced PD Mouse Model

Six-week-old male C57BL/6 mice (20–22 g; DBL, Korea) were divided into two groups: the control group and MPTP-treated group (MPTP). In the control group, mice were injected intraperitoneally with 100 μL phosphate-buffered saline (PBS) once a day for 4 weeks, while in the MPTP group, mice were injected intraperitoneally with MPTP-HCL (20 mg/kg of free base; Sigma, United States) in PBS (100 μL) every 24 h for 4 weeks to produce a semi-chronic model of PD. On the day after the final MPTP treatment, mice were anesthetized using Alfaxan and perfused transcardially with cold PBS for western blotting. The MPTP model of PD shows dopaminergic cell death in the SN (Choi and Lim, 2010; Yeo et al., 2013). All animal experiments conducted for this study were approved by the Sang Ji University Animal Experimentation Committee.