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2. Assessment of sperm motility by computer-assisted semen analysis

SS Seung-Hun Song
DS Dong Hyuk Shin
YH Young Sun Her
MO Mi Hee Oh
JB Jung Won Baek
SS Suye Sung
JE Jin Hee Eum
YH Yohan Heo
DK Dong Suk Kim
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Sperm motility was measured by using computer-assisted semen analysis (CASA; Sperm Class Analyzer; version evolution 6.0.0.1; Microptic, Barcelona, Spain) according to the WHO classification. An external quality-control service (SCA Maintenance; Microptic, Barcelona, Spain) was performed for this study. Sperm motility was classified into 4 groups. (grade A: fast progressive, grade B: slow progressive, grade C: nonprogressive, grade D: immotile). The CASA settings were according to the manufacturer's instructions. Each reported measurement was an average of five different captured fields. Total motility (A+B+C), progressive motility (A+B), and grade A sperm after in vitro exposure to a PDE5 inhibitor for 0, 30, 60, and 120 minutes were measured.

Copyright and License information: The Korean Urological Association, ©2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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