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Each mouse was tested for forelimb strength using the wire hanging test. Prior to testing, mice were trained and acclimatized by placing them on the wire of the hanging bar apparatus for several min. The test can be used to measure decline in strength and neuromuscular function with age and consisted of measuring the time before the mouse falls from the hanging wire. The test was repeated two more times with approximately 30 min break between trials for each mouse.
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