Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Treatment of cells with Rac1 inhibitors
Request a Protocol
Ask a
question
Favorite
To inhibit Rac1 activity, cells were treated for 60–120 min with Rac1 inhibitor NSC23766 (50 μM; Bio-Techne Corporation, Minneapolis, MN) or microinjected with recombinant dominant-negative mutant Rac1 protein (Ridley et al., 1992 
) (needle concentration 5 mg/ml) as described in Waterman-Storer et al. (1999) .
Copyright and License information: ©2021 Burakov, Vorobjev, “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
