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β-Carotene/linoleic acid bleaching method

AS Ahmed Snoussi
IE Ismahen Essaidi
HK Hayet Ben Haj Koubaier
HZ Houda Zrelli
IA Ibrahim Alsafari
TŽ Tesic Živoslav
JM Jelena Mihailovic
MK Muhummadh Khan
AO Abdelfatteh El Omri
TV Tanja Ćirković Veličković
NB Nabiha Bouzouita
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The antioxidant activity of EEs was estimated through β-carotene bleaching method as described by Suja et al. [28]. Briefly, 0.2 mg β-carotene, 20 mg linoleic acid and 200 mg Tween 40 were mixed in chloform. Then the mixture was concentrated by evaporating the solvent. The obtained residue was emulsified in 50 ml oxygenated distilled water and aliquoted. Finally, 0.2 mL of EEs (0.2 mg/ml), ethanol and BHT respectively for negative and positive controls were added to aliquots (4 mL) of the β-carotene/linoleic acid emulsion, and heated at 50 °C, in a water bath, in order to accelerate the oxidation. The absorbance was measured at 470 nm until the discoloration of the control solution (t = 120 min).

Antioxidant activity percentages (AA%) were calculated using the following equation:

where, AS (120) is the absorbance of the tested sample at 120 min, AC (120) is the absorbance of the control at 120 min and AC (0) is the absorbance of the control at 0 min.

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