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Gel bands of proteins were excised for in-gel digestion, and proteins were identified by mass spectrometry. Briefly, the gel bands were reduced with 25 mM of dithiothreitol (DTT) and alkylated with 55 mM Iodoacetamide. In-gel digestion was performed using Pepsin (Promega) in 1% formic acid at 37 °C overnight. The peptides were extracted twice with 1% trifluoroacetic acid in 50% acetonitrile aqueous solution for 30 min. The peptide extracts were then centrifuged in a SpeedVac to reduce the volume.
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