Tumors and Patient Cohorts for EMSA Studies, Transcriptomic and Overall Survival Analyses

Clinical data of the patients studied are presented in Supplementary Tables S1 and S2 and in Supplementary Materials and Methods . Tumors were diagnosed according to the World Health Organization classification (17). The study was performed with approval of an Institutional review board and written informed consent was obtained from all participants at the time of enrollment. All tumor samples were reviewed for tumor infiltration, and selected cases were those for which tumor infiltration was over 90%. EMSA and transcriptomic analysis (HGU133 +2.0 Affymetrix Gene Chip microarray; accession number {"type":"entrez-geo","attrs":{"text":"GSE87371","term_id":"87371"}}GSE87371; Supplementary Table S3 ) were performed on frozen material (see Supplementary Materials and Methods for details).

Survival and Cox multivariate analysis was done with the Survival package (URL: https://github.com/therneau/survival). Overall survival (OS) was evaluated from the date of enrollment to the date of death from any cause. C-Harrel concordance index was calculated as described (18). Details are given in the Supplementary Materials and Methods .