Bisulfite conversion of DNA, restoration and array processing

SV Sandra Ferreyra Vega
TB Thomas Olsson Bontell
AC Alba Corell
AS Anja Smits
AJ Asgeir Store Jakola
HC Helena Carén
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Between 500 and 1000 ng of extracted DNA was bisulfite-converted with the EZ DNA methylation kit (Zymo) and restored with the Infinium HD FFPE Restore Kit (Illumina), according to the instructions supplied by the manufacturer. The Infinium MethylationEPIC BeadChip array (Illumina) was used to generate genome-wide DNA methylation profiles for the tumor samples (UCL Genomics, UK).

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