Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
HeLa cells were subjected to two rounds of transfection (days 0 and 2) with siRNA using Oligofectamin (Life Technologies) according to the manufacturer's protocol. For additional transient overexpression, plasmids were transfected on day 4 using X-tremeGENE 9 DNA transfection reagent (Roche). For silencing, the following siRNA was used: AP-2α 5′- AAGAGCAUGUGCACGCUGGCCA. Scrambled AP-2μ sequence 5′-GTAACTGTCGGCTCGTGGTTT was used as control siRNA.
Copyright and License information: ©2017, The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
