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2.4. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and western blotting
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The identity of EVs was established by the presence of specific surface proteins (CD9 and CD63), and the ability to remove the urinary non‐EV‐associated proteins using ultracentrifugation with a 30% sucrose/D2O cushion, evaluated by the absence of THP. Details are provided in Materials and Methods (Supporting Information).
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