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Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)

YS Yousra Shafiq
SN Syed Baqir Shyum Naqvi
GR Ghazala H. Rizwani
MA Muhammad Arif Asghar
RB Rabia Bushra
SG Sana Ghayas
AR Ahad Abdul Rehman
MA Muhammad Asif Asghar
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MICs and MBCs of NOEE against tested isolates were determined using the broth dilution method [25]. Briefly, the plant extract with a concentration of 50 mg/mL was prepared in a nutrient broth and make several dilutions up to 0.1 mg/mL using the serial dilution method. All tested bacterial strains were adjusted to the concentration of 1 × 106 cfu/mL as McFarland standard. After overnight incubation of inoculated microbial plates, optical densities (ODs) were measured using an ELISA reader (Infinite 200; USA) at 600 nm. In addition, MBCs of plant extract was determined by spreading the solution of microbial strains on nutrient agar plates and incubated at 37 °C for 24 h. After an incubation period, bacterial colonies on each inoculated plate were observed [26, 27]. Each experiment was performed three times and mean ± SD values of each test were reported.

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