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HAFFNER’s tail clip method

MG Mennatallah A Gowayed
AA Amany Abdel-Bary
RE Rasha A El-Tahan
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An artery clip was applied to the root of the tail of rat (approximately 1 cm from the body) to induce pain [23] and the reaction time was noted. The animal quickly responded to this noxious stimulus by biting the clip or the tail near the location of the clip. The time between stimulation onset and response was measured by a stopwatch in 1/10 seconds increments. In all groups, tail clip test was performed preceding drug administration, and at 15, 30, 45- and 60-minutes following drug administration. The test latency (reaction time) at each time interval was calculated. The % analgesia was calculated (% analgesia = MPE = (TL – BL / ML – BL) *100), where MPE = max possible effect, TL = test latency, BL = control latency or basal latency and ML = max latency or cut off time. Cut off time of ten seconds was obliged in all sets of experiments [24] .

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