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Total RNA was obtained using an RNA extraction kit (#RN001; ESscience, Shanghai, China) and converted to cDNA according to the manufacturer's instructions (#RR036A‐1; TAKARA, Japan). The relative expressions of identified genes were performed and measured by quantitative real‐time PCR assays (ABI Q6 System, Applied Biosystems). All the genes were normalized to GAPDH, and the fold change was calculated as 2–ΔΔCT comparative thresholds. Specific primer sequences are shown in Table S1.
Copyright and License information: ©2021 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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