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3D Structured illumination imaging

LL Li Li
HL Hui Liu
PD Peng Dong
DL Dong Li
WL Wesley R Legant
JG Jonathan B Grimm
LL Luke D Lavis
EB Eric Betzig
RT Robert Tjian
ZL Zhe Liu
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After saturated JF549-HTL labeling, Htt-94Q-CFP/H2B-HaloTag or Htt-94Q-CFP/HaloTag-Sp1 ES cells on the cover glasses were placed on a home-build 3D SIM microscope with environment control (37°C). Two color live-cell 3D SIM experiments were performed as previously described (Fiolka et al., 2012), except that two color data were acquired on a plane-by-plane rather than a volume-by-volume basis to improve the local registration of the two imaging channels within the moving live cell.

Copyright and License information:  ©2016, Li et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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