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Proliferation Analysis by Phospho-Histone H3 (pH3) Immunostaining

KS Kai O. Skaftnesmo
DC Diego Crespo
LK Lene Kleppe
EA Eva Andersson
RE Rolf B. Edvardsen
BN Birgitta Norberg
PF Per Gunnar Fjelldal
TH Tom J. Hansen
RS Rüdiger W. Schulz
AW Anna Wargelius
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In order to assess germ cell proliferation, paraffin-embedded testis tissue was sectioned at 4 μm thickness and then subjected to an immunocytochemical procedure to detect the endogenous proliferation marker phospho-histone H3 (pH3). Paraffin sections were deparaffinized and rehydrated according to standard protocols and subjected to pH3 immunohistochemistry using a polyclonal rabbit anti-human pH3 (Ser10) antibody (Upstate®; Sigma-Aldrich) as previously described (Almeida et al., 2008), except that the primary antibody was detected by undiluted HRP-conjugated goat anti-rabbit IgG (Brightvision Immunologic).

Copyright and License information:  ©2021 Skaftnesmo, Crespo, Kleppe, Andersson, Edvardsen, Norberg, Fjelldal, Hansen, Schulz and Wargelius.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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