Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Syneresis measurement

RA Raphael Lucas Jacinto Almeida
TP Tamires dos Santos Pereira
RA Renata Duarte Almeida
ÂS Ângela Maria Santiago
WM Wanda Izabel Monteiro de Lima Marsiglia
EN Elizabeth Harumi Nabeshima
LC Líbia de Sousa Conrado
RG Rennan Pereira de Gusmão
request Request a Protocol
ask Ask a question
Favorite

Syneresis index capacity was determined according to the methodology proposed by43, with modifications performed for all gel formulations (SN, MS1, MS2, SN + JPP, MS1 + JPP, and MS2 + JPP) and followed for 28 days at 7-day intervals for each reading, as follows: (D0, D7, D14, D21, and D28).

Three days after the gel preparation and storage at 6 °C, the samples (15 g) were centrifuged at 3000 g for 10 min under refrigeration at 6 °C, in duplicate. The water mass separated from the gel network during centrifugation, divided by the initial gel weight and multiplied by 100, according to Eq. (4), calculated the percentage of syneresis.

Mw is the water's weight separated from the gel after centrifugation (g), and Mi is the initial gel weight (g).

Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A