Chromatin isolation using RNA purification assay (ChIRP)

YL Yang Liu
TT Tianchi Tang
XY Xiaosheng Yang
PQ Peng Qin
PW Pusen Wang
HZ Huiping Zhang
MB Min Bai
RW Rong Wu
FL Fan Li
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Chromatin isolation using RNA purification (ChIRP) was performed with a ChIRP Kit (BersinBi, Guangzhou, China) according to the suggested protocol. 3′ end Biotin-triethylene glycol-modified DNA probes targeting LINC01133 were constructed and synthesized. Trizol (Invitrogen, Carlsbad, CA, USA) was used to collect total RNA, and protein expression was analyzed by western blot. The sequences of the biotin probes are available in Supplementary Table 3.

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