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Purified proteins cleaved from their tags were prepared. CD spectra were collected with a thermostated cell holder on a J-1500 spectropolarimeter (Jasco, Tokyo) at different temperatures (20–100°C). A quartz cell with a 1 cm path length was used for measurements in the far-UV region from 200 to 240 nm. The spectra of buffer alone were used to correct the sample spectra. Results were analyzed using Excel (Microsoft) and GraphPad Prism (GraphPad Software).
Copyright and License information: ©2021 Lin, Wang, Ren, Xia, He and Lu.
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